IMPACT OF MICROBIOME ENGINEERING ON GUT-BRAIN AXIS MODULATION AND BEHAVIORAL DISORDERS IN CANINES: A PRECISION VETERINARY MEDICINE PERSPECTIVE

Abstract

The microbiota-gut-brain axis represents a critical communication network linking gastrointestinal microbiota with central nervous system function. This study investigated the potential of microbiome engineering to improve behavioral outcomes in canines exhibiting anxiety and related disorders. A mixed-methods experimental design was employed involving behavioral assessments, microbial diversity profiling via 16S rRNA sequencing, and targeted interventions including probiotics, prebiotics, and fecal microbiota transplantation. Behavioral scores and microbial indices were analyzed pre- and post-intervention across 40 dogs. Results showed a statistically significant decrease in anxiety scores post-intervention, particularly in groups receiving Lactobacillus rhamnosus and mixed probiotic formulations. Enhanced microbial alpha diversity and improved behavioral phenotypes were strongly correlated (p < 0.01), reinforcing the gut-brain axis hypothesis. Hybrid visualizations, including bar-line overlays and PCoA clustering, further revealed meaningful microbial shifts. This study provides strong evidence that modulating the gut microbiome through personalized interventions can improve mental health in dogs. The findings underscore the translational relevance of gut-brain axis manipulation for veterinary psychiatric care and support the development of precision-based therapeutic strategies in companion animals.